4 parameters in typical biological systems of curve fitting are:
(1) TOP (highest value) -- This could be dervied from using an asymptote value
(2) BOTTOM (lowest value) -- This could be dervied from using an asymptote value
(3) SLOPE of the line connecting top and bottom (Often used as Hill Slope)
(4) CONSTANT or EC50 value
Use Graphpad for ease. Academic license and student licenses are, while not affordable in Asian countries,affordable for Western Europe, Canada and US.
Graphpad should do something for allowing more users in Asian countries make software available for day's salary (3 dollars), otherwise Rapidshare is easy way to get access to Graphpad (or other software).
I do support software piracy on economic status mitigation.