You may be right. I'll have to check it out. It's been a while since I
visited that problem and maybe I didn't understand it well enough at the
time. But I recall that with the 2450's Smart Panel it saved the same size
file whether I used 48 or 24 bits in the TWAIN, and don't remember getting
that warning/OK popup. I had been looking to save 48 bit scans (or 16 bit
greyscale) for possible future use (when I might afford full Photoshop) of
materials that had to be returned to their owners, and recall being blocked
from that. Maybe the problem was that it was prints and not transparencies?
Running an experiment
48 bit color photo, 300 dpi, 8.5x11.7 inches, Twain claims 51.21 MB
close, save as TIFF --- size 26,223 KB
color photo, ...the same... Twain says 25.60 MB
close, save TIFF --- size 26,223 KB
I can't remember all the details of where I found the info that said (or
that I remember saying) you had to save 48-bit from within an application.
I should check the epson site to see if there's an update.