Overlay Challenge

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I want to superimpose a graph generated from a CSV file onto an microscopic live picture. Although I am able to do this at a particular zoom level as I am changing the zoom there is a mismatch between the graphic picture generated and the live picture from the mic.

Can someone please tell me if there is a good way to do this. Please write back to me at (e-mail address removed)
 

Silverhazesurfer

Master of Logic
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first thing that comes to mind is photoshop and transparency. what are you using now to accomplish what you have thus far?
 

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