I think the limit is the size of the sheet you are working on (65,532
rows and 256 columns in Excel 2003 and before). However, with a
validation list it would take a long time to scroll through to select
the item you want if you have such a big range - could you not
organize the data (eg alphabetically) and make use of dependent data
validation? Debra Dalgleish shows how here:
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